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6%) ended up categorized as advantageous, in which just about every single mutation was preferred around the wild-sort residue. In comparison, the quantity of effective positions lessened to ten in +Lon assortment, with only a few surface-uncovered positions (E48, T68, D127) popular amongst The 2 advantageous sets. at the same time, the number of Restricted positions increased from forty two to 67 Along with the reintroduction of Lon into the choice strain (Figure 2C). These final results support the conclusion that Lon activity broadly penalizes mutations, which include a substantial subset of the useful mutations. Overall, the changes upon modulating Lon action produce a design by which upregulating Lon increases constraints on DHFR, along with the mutational landscape variations from staying permissive when Lon is absent to being more restricted when Lon is present (Figure 2nd).
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